第5回　配偶子制御セミナー

“GERMLINE DEVELOPMENT: FORMOSA ANTIBODY AND THE STORIES THEREAFTER”
講師　　Dr. Chun-che Chang　（張俊哲 博士）
所属　　National Taiwan University
日時　　平成21年7月9日（木）16時−17時
場所　　自然科学研究機構　岡崎統合バイオサイエンスセンター　山手９階セミナー室B

講演の内容
Chun-che Chang博士は、"evo-devo"の視点からショウジョウバエ以外の昆虫の生殖細胞発生を
遺伝子レベルで研究されています。
今回は、バッタやアブラムシなどのvasa・nanos遺伝子の研究を中心としたお話を伺えると思います。

In animals germ cells are specified during embryogenesis;
this specification relies either on a maternally inherited germ plasm in early development
or induction signals from neighboring somatic cells in mid or late development.
Whether the modes of germline specification are associated with embryonic pattern formation
or with phylogenetic relationship is still not clear.
Nevertheless, it has been demonstrated that many animals utilize two highly conserved germline genes,
vasa and nanos, for germline development regardless which specification mode they employ.
In order to examine the diversity of germline specification in insects,
we produced a highly cross-reacting antibody formosa (for most Vasa)
that recognize conserved Vasa-specific motifs in many species.
In this presentation I shall describe how we take advantage of such an antibody
to explore germline specification in the grasshopper Schistocerca gregaria
and the pea aphid Acyrthosiphon pisum, two hemimetabolous insects
whose body plan is determined differently from the model holometabolous insect Drosophila melanogaster.
In addition, I will present our recent findings concerning the establishment of egg polarity
and the migration of germ cells in the pea aphid.
Taken together, these data allow us to shed new light on germline development in insects.

参考文献
Ting-Yu Huang, Charles E. Cook, Gregory K. Davis, Shuji Shigenobu, Rita P.-Y. Chen, and Chun-che Chang
(2009) Anterior development in the parthenogenetic and viviparous form of the pea aphid,
Acyrthosiphon pisum: hunchback and orthodenticle expression. Insect Mol. Biol. (submitted)

Chun-che Chang, Ting-Yu Huang, Charles E. Cook, Gee-Way Lin, Chun-Liang Shih, and Rita P.-Y. Chen
(2009) Developmental expressionof Apnanos during oogenesis and embryogenesis
in the parthenogenetic pea aphid Acyrthosiphon pisum (Hemiptera: Aphidoidea).
Int. J. Dev. Biol. 53: 169-176.

Taro Mito, Taro Nakamura, Isao Sarashina, Chun-che Chang, Shotaro Ogawa, Hideyo Ohuchi, and Sumihare Noji
(2008) Dynamic expression patterns of vasa during embryogenesis in the cricket Gryllus bimaculatus.
Dev. Genes Evol. 218: 381-387.

Chun-che Chang, Ting-Yu Huang, Chun-Liang Shih, Gee-Way Lin, Te-Pin Chang, Hui Chiu, Wen-Chang Chang
(2008) Whole-mount identification of gene transcripts in aphids:
Protocols and evaluation of probe accessibility. Arch. Insect Biochem. Physiol. 68: 186-196.

Chun-che Chang, Hui Chiu, and How-Jing Lee (2008) Identification of nuage-like structures
in the panoistic ovarioles of the German cockroach Blattella germanica (Linnaeus).
Formosan Entomologist 28: 1-10. (cover article)

Chun-che Chang, Gee-way Lin, Charles E. Cook, Shwu-bin Horng, How-jin Lee and Ting-yu Huang
(2007) Apvasa marks germ-cell migration in the parthenogenetic pea aphid Acyrthosiphon pisum (Hemiptera: Aphidoidea).
Dev. Genes Evol. 217: 275-287.

Chun-che Chang, Wen-chih Lee, Charles E. Cook, Gee-way Lin, and Tschining Chang
(2006) Germ-plasm specification and germline development in the parthenogenetic pea aphid Acyrthosiphon pisum:
Vasa and Nanos as markers. Int. J. Dev. Biol. 50: 413-421.

Chun-che Chang, Peter Dearden, and Michael Akam (2002) Germline development
in the grasshopper Schistocerca gregaria: Vasa as a marker. Dev. Biol. 252, 110-118. (cover article)

問い合わせ ;
自然科学研究機構 岡崎統合バイオサイエンスセンター　
小林悟　(skob@nibb.ac.jp)
重信秀治　(shige@nibb.ac.jp)
℡:0564-59-5875