| library | Host strain | vector backbone | 5' primer | 3' primer | cap signature seq (end of non-template) | seq after polyA | |||
| pph | cap-trapper | bluescript with a loxP site(excise | M13-21 or T7 | reverse or T3 | acagcgatgactcgagaaccg | ctctccaggg ttggatccgg ccataagggc ctgatccttc | |||
| pphb | cap-trapper | bluescript with a loxP site(excise | M13-21 or T7 | reverse or T3 | acagcgatgactcgagaaccg | ctctccaggg ttggatccgg ccataagggc ctgatccttc | |||
| pphn | cap-trapper | bluescript with a loxP site(excise | M13-21 or T7 | reverse or T3 | acagcgatgactcgagaaccg | ctctccaggg ttggatccgg ccataagggc ctgatccttc | |||
| pphf | v-capping | DH12S | pGCAPzf3 | M13-21 or T7 | reverse | gggcgaattggcggccaaatcggccgaatt | ggccataagggccagcttgagtattctatagtg | ||
| ppsp | oligo-capping | DH5alpha | pME18S-FL3 (AB009864) | SP5 | SP3M | cgagcactgt tggcctactg g | ggccacatgt gctcgagctg caggtcgcgg ccgctagact agtctagaga |
||
| ppls | oligo-capping | pME18S-FL3 (AB009864) | SP5 | SP3M | cgagcactgt tggcctactg g | ggccacatgt gctcgagctg caggtcgcgg ccgctagact agtctagaga | |||
| ppaa | oligo-capping | pME18S-FL3 (AB009864) | SP5 | SP3M | cgagcactgt tggcctactg g | ggccacatgt gctcgagctg caggtcgcgg ccgctagact agtctagaga | |||
| ppgs | oligo-capping | pME18S-FL3 (AB009864) | SP5 | SP3M | cgagcactgt tggcctactg g | ggccacatgt gctcgagctg caggtcgcgg ccgctagact agtctagaga | |||
| primer | |||||||||
| M13-21 | tgtaaaacgacggccagt | ||||||||
| Reverse | agcggataacaatttcacacagg | ||||||||
| SP5 | cttctgctctaaaagctgcg | ||||||||
| SP3M | tgtgggaggttttttctcta | ||||||||
| T3 | aattaaccctcactaaaggg | ||||||||
| T7 | taatacgactcactataggg | /(alternative) gtaatacgactcactatagggcga | |||||||