More than 80% of land plant families have symbiotic relationships with arbuscular mycorrhizal (AM) fungi. AM fungi absorb minerals, including phosphate, from the soil and provide them to the plants. The origin of AM symbiosis is thought to have been in the early Devonian period. On the other hand, the root nodule symbiosis that occurs between legumes and rhizobial bacteria, unlike AM symbiosis, involves host-specific recognition and postembryonic development of a nitrogen-fixing organ. Despite marked differences between the fungal and bacterial symbioses, common genes required for both interactions have been identified using model legumes; Lotus japonicus and Medicago truncatula. Our laboratory, which was launched in 2009, focuses on the early stages of the interaction between these microorganisms and L. japonicus in order to shed light on the nature of ancient plant functions involved in symbiosis signaling.
This division is currently recruiting graduate students.
Suzaki, T., Yoro, E., and Kawaguchi, M. (2015). Leguminous plants: Inventors of root nodules to accomodate symbiotic bacteria. Int. Rev. Cell Mol. Biol. 316, 111-58
Takeda, N., Handa, Y., Tsuzuki, S., Kojima, M., Sakakibara, H., Kawaguchi, M. (2015). Gibberellins interfere with symbiosis signaling and gene expression and alter colonization by arbuscular mycorrhizal fungi in Lotus japonicus. Plant Physiol. 167, 545-57.
Sasaki, T., Suzaki, T., Soyano, T., Kojima, M., Sakakibara, H., and Kawaguchi, M. (2014). Shoot-derived cytokinins systemically regulate root nodulation. Nat. Commun. 5, 4983
Soyano, T., Hirakawa, H., Sato, S., Hayashi, M., and Kawaguchi, M. (2014). Nodule inception creates a long-distance negative feedback loop involved in homeostatic regulation of nodule organ production. Proc. Natl. Acad. Sci. USA 111, 14607-12
Suzaki, T., Ito, M., Yoro, E., Sato, S., Hirakawa, H., Takeda, N., and Kawaguchi, M. (2014). Endoreduplication-mediated initiation of symbiotic organ development in Lotus japonicus. Development 141, 2441-5
Yoro, E., Suzaki, T., Toyokura, K., Miyazawa, H., Fukaki, H., and Kawaguchi, M. (2014). A positive regulator of nodule organogenesis, NODULE INCEPTION, acts as a negative regulator of rhizobial infection in Lotus japonicus. Plant Physiol, 165, 747-58
Okamoto, S., Shinohara, H., Mori, T., Matsubayashi, Y., and Kawaguchi, M. (2013). Root-derived CLE glycopeptides control nodulation by direct binding to HAR1 receptor kinase. Nat. Commun. 4, 2191
Suzaki, T., Yano, K., Ito, M., Umehara, Y., Suganuma, N., and Kawaguchi, M. (2012). Positive and negative regulation of cortical cell division during root nodule development in Lotus japonicus is accompanied by auxin response. Development 139, 3397-4006
Fujita, H., Toyokura, K., Okada, K., and Kawaguchi, M. (2011). Reaction-diffusion pattern in shoot apical meristem of plants. PLoS One 6, e18243
Miyazawa, H., Oka-Kira, E., Sato, N., Takahashi, H., Wu, G. J., Sato, S., Hayashi, M., Betsuyaku, S., Nakazono, M., Tabata, S., Harada, K., Sawa, S., Fukuda, H., and Kawaguchi, M. (2010). A receptor-like kinase, KLAVIER, mediates systemic regulation of nodulation and non-symbiotic shoot development in Lotus japonicus. Development 137, 4317-25
Okamoto, S., Ohnishi, E., Sato, S., Takahashi, H., Nakazono, M., Tabata, S., and Kawaguchi, M. (2009). Nod factor/nitrate-induced CLE genes that drive HAR1- mediated systemic regulation of nodulation. Plant Cell Physiol. 50, 67-77
Saito, K., Yoshikawa, M., Yano, K., Miwa, H., Uchida, H., Asamizu, E., Sato, S., Tabata, S., Imaizumi-Anraku, H., Umehara, Y., Kouchi, H., Murooka, Y., Szczyglowski, K., Downie, JA., Parniske, M., Hayashi, M., and Kawaguchi, M. (2007). NUCLEOPORIN85 is required for calcium spiking, fungal and bacterial symbioses and seed production in Lotus japonicus. Plant Cell 19, 610-24
Nishimura, R., Hayashi, M., Wu, G-J., Kouchi, H., Imaizumi-Anraku, H., Murakami, Y., Kawasaki, S., Akao, S., Ohmori, M., Nagasawa, M., Harada, K., and Kawaguchi, M. (2002). HAR1 mediates systemic regulation of symbiotic organ development. Nature 420, 426-9
Kawaguchi, M. (2000). Lotus japonicus 'Miyakojima' MG-20: An early flowering accession suitable for indoor handling. J. Plant Res. 113, 507-9
Professor KAWAGUCHI, Masayoshi TEL: +81 564 55 7564 E-mail: email@example.com