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National Institutes of Natural Sciences

National Institute for Basic Biology

International Cooperation

Practical Courses - Conference

The 6th NIBB International Practical Course and The 1st NIBB - TLL Joint International Practical Course "Developmental Genetics of Medaka IV"

Organizers Kiyoshi Naruse (NIBB)
Venue National Institute for Basic Biology
Date Nov. 14-21, 2011
Link Website (http://www.nibb.ac.jp/course/)
Poster The 6th NIBB International Practical Course and The 1st NIBB - TLL Joint International Practical Course

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The 6th International practical course "Developmental Genetics of Medaka IV " was held in the dedicated National Institute for Basic Biology Bioscience training course rooms from November 14th the to 21st , 2011. This time the course was held together with the Temasek Life Science Institute (Singapore )  as "The 1st NIBB TLL Joint International Practical Course".

Associate Professor C. Winkler from Singapore participated as an invited lecturer. Six lecturers from Japan, Dr. Minoru Tanaka (National Institute for Basic Biology), Dr. Rie Kusakabe (Kobe University), Dr. Jun Kitano (National Institute of Genetics), Dr. Yoshihito Taniguchi (Keio University), Dr. Shinya Minori (National Institute of Genetics), and Dr. Tomonori Deguchi (National Institute of Advanced Industrial Science and Technology) were in charge of the lectures and hands-on training.

Sixteen Students from nine countries, the United States , South Korea , Norway , Canada , Italy , India , Singapore , China , and Japan participated in the course.

Students trained in creating and using frozen sperm for artificial insemination, production of transgenic medaka using BAC clones, the local gene expression control system IR-LEGO (infrared laser), ENU treatment mutant creation, as well as learning the reverse genetic approach by TILLING library screening with the High Resolution Melting (HRM) analysis. It was a pretty hard schedule and included seminars held by lecturers every day.

On the other hand, on Sunday all the course participants visited the "Medaka of the World" exhibit at the Higashiyama Zoo & Botanical Gardens and conducted facility tours, even including the back yard. It was a fulfilling day covering areas outside the normal rigors of training.  

The next course will be held in Singapore from July 22nd to the 31st, 2012.


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Program

Mon, November 14th, 2011
Arrival and Registration
16:00 - 16:30 Opening Remarks & Orientation
16:30 - 17:30 Seminar by Dr. Winkler, Christoph (National Univ. of Singapore, Singapore)
18:00 - 20:00 Welcome Party   
 
Tue, November 15th, 2011

09:00 - 10:00 Seminar by Dr. Tanaka
10:00 - 10:30 Outline for Generation of BAC transgenics by Dr. Takehana
10:30 - 12:00 PCR for Target Amplification
13:00 - 16:30 Target Gene Treatment with RE
              Target Gene Purification
              DY380 preculture
16:30 - 17:30 Seminar by Dr. Kusakabe, Rie (Kobe Univ.)
18:00 - 20:00 Social Hour
   
Wed, November 16th, 2011

09:00 - 12:00 Starting Large Culture for Competent Cells
13:00 - 16:30 Heat Induction (DY380)
              Competent Cell Prep/Electroporation
16:30 - 17:30 Seminar by Dr. Kitano, Jun (NIG)
18:00 - 20:00 Social Hour
   
Thu, November 17th, 2011

09:00 - 09:30 Outline for Procedure of Cryopreservation by Dr. Sasado
09:30 - 12:00 Dissection of Testis
              Sperm Freezing
13:00 - 13:30 Outline for Procedure of Insemination by Dr. Sasado
13:00 - 16:00 Insemination/Obserbation
16:30 - 17:30 Colony pick/Culture for BAC purification
   
Fri, November 18th, 2011
 09:00 - 09:30 Outline for Microinjection by Dr. Kimura
09:30 - 12:00 Microinjection (practice)
13:00 - 16:30 Recombinant BAC purification
16:30 - 17:30 Seminar by Dr. Winkler, Christoph (National Univ. of Singapore, Singapore)
   
Sat, November 19th, 2011
 09:00 - 11:00 Microinjection/Obervation
11:00 - 12:00 Seminar by Dr. Taniguchi, Yoshihito (Keio Univ.)
13:00 - 13:30 Outline for TILLING by Drs. Kitano/Hashimoto
              Outline for IR-LEGO by Dr. Kamei
13:30 - 18:00 TILLING/IR-LEGO
   
Sun, November 20th, 2011
   
Mon, November 21st, 2011
 09:00 - 10:00 Seminar by Shinya, Minori (NIG)
10:00 - 10:30 Outline for ENU mutagenesis by Dr. Deguchi
10:30 - 12:00 ENU mutagenesis
13:00 - 13:30 Outline for TILLING by Drs. Kitano/Hashimoto
              Outline for IR-LEGO by Dr. Kamei
13:30 - 18:00 TILLING/IR-LEGO
18:30 - Farewell Party at a Japanese Restaurant