National Institute for Basic Biology
| Organizers | Robert E. Braun (The Jackson Laboratory) Mark Van Doren (The Jackson Laboratory) Satoru Kobayashi (NIBB) Shosei Yoshida (NIBB) |
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| Venue | Okazaki Conference Center, Okazaki, Japan |
| Date | Jul. 17-21, 2012 |
| Link | Official Website （http://www.nibb.ac.jp/conf58/） |
| Poster |
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The 58th and 60th NIBB Conference "Germline -Specification, Sex, and Stem Cells-" was held from July 17th to 21st at the Okazaki Conference Center. Originally the 58th NIBB conference on "gamete stem cells" was to be held on in July, 2011, however, due to the March 11th earthquake and tsunami the meeting was postponed. Fortunately, the 60th NIBB conference on "germline development" was planned for this year and we were able to hold this unprecedented “58th and 60th ‘joint’ NIBB conference”.
At the symposium we focused on three subjects central to germline research, the formation of the germline, the sexual characteristics of the germline, and gamete stem cells, and thoroughly discussed the universal and distinctive aspects of a wide range of species, including mammals (primates, mice), birds, fish, ascidians, hydra, nematodes, planarians, and fruit flies. Over 130 participants from around the world took part, with four scientists who are pushing the bounds of present research giving special lectures based on data from the cutting edge of this field, which lead to impassioned debate from the beginning to the end of the conference.
Following the special lectures, lectures on the mechanisms regulating specification and sexual development of the germline continued. The topics included identification of the genes that induce feminization or masculinization of primordial germ cells, somatic control of germline sex, and the regulatory mechanisms of gene expression in germline cells. Research using birds, planarians, ascidians, and hydra was also presented, allowing for participants to share the invaluable viewpoint of evolutional conservation and divergence of the regulatory mechanism underlying germline development, and to feel the true breadth of the germline research field.
On the following days, researchers investigating the stem cells of gametogenesis in a variety of animals, including mice, primates, fish and fruit flies, were able to come together and discuss their research in a single venue, making this a historic opportunity. Many debates were presented based on similarities in the gamete stem cell systems in mice, fruit flies, and other animals displaying mutual differences in gonadal morphology, as well as computer simulations and quantitative statistical analyses of stem cell dynamics. A variety of revolutionary germ cell manipulating techniques motivated by medical science, animal husbandry, and fisheries science was another important theme of the conference, with much lively debate on the subject to be had.
Though the schedule for the whole event was rather tight, we believe the conference provided ample ground for participants to meet between conferences, and at meals and other get-togethers, and to strengthen and broaden their personal bonds. It is our hope that these new connections might become the seeds for new breakthrough research in the field.
Finally, we would like to give our thanks to all the participants for this year's conference for attending, the Office of International Cooperation and the members of the organizing research laboratories for managing the conference, Grant-in-Aid for Scientific Research on Innovative Areas, “Regulatory Mechanisms of Gamete Stem Cells” from the Ministry of Education, Culture, Sports, Science, and Technology for co-sponsoring the event, and to the Inoue Foundation for Science and the Daiko Foundation for their support.
Organizers: Robert. E. Braun (USA, The Jackson Laboratory), Mark Van Doren (USA, Johns Hopkins University), Satoru Kobayashi (National Institute of Basic Biology), Shosei Yoshida (National Institute of Basic Biology)
| July 17th (Tue) at the Okazaki New Grand Hotel | |
| 19：00 - | A reception for invited speakers |
| July 18th (Wed) at the Okazaki Conference Center (OCC) | |
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The 60th NIBB Conference
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| Opening Remarks | |
| 09:00 - 09:10 | Kiyotaka Okada (Director-General of the National Institute for Basic Biology, Japan) |
| 09:10 - 09:20 | Satoru Kobayashi & Shosei Yoshida (National Institute for Basic Biology, Japan) |
| Special Lectures | |
| Mark Van Doren, Satoru Kobayashi | |
| 09:20 - 10:20 |
“Silencing the Germline” Ruth Lehmann (New York University, USA) |
| 10:20 - 10:30 | Coffee Break |
| 10:30 - 11:30 |
“Development in vivo of Fertilization-Competent Oocyte-like Cells in the Absence of Meiotic Initiation or Prophase” David C Page (Whitehead Institute for Biomedical Research, USA) |
| 11:30 - 13:00 | Lunch Break |
| Session I | Regulatory Mechanism of Germline Specification |
| Akira Nakamura, Geraldine Seydoux | |
| 13:00 - 13:40 |
“RNA Silencing in Germline Stem Cells” Geraldine Seydoux (Johns Hopkins University, USA) |
| 13:40 - 14:20 |
“Germline Transcriptional Repression in Drosophila and Ciona Embryos” Akira Nakamura (RIKEN Center for Developmental Biology, Japan) |
| 14:20 - 14:40 | Coffee Break |
| 14:40 - 15:20 |
“PEM, a Localized Maternal Factor Unique to Ascidians, Represses Germline Gene Expression through RNAP II Regulation and Binding to p-TEFb” Gaku Kumano (Osaka University, Japan) |
| 15:20 - 16:00 |
“Mechanism and Reconstitution In Vitro of Germ Cell Specification in Mice” Mitinori Saitou (Kyoto University, Japan) |
| 16:00 - 16:30 | Coffee Break |
| Short Talks I | |
| Yasuhisa Matsui, Mitinori Saitou | |
| 16:30 - 16:45 |
“Primordial Germ Cells Transmigrate from Blood Stream to Gonad in Avian Embryos: Novel Behavior Revealed by Live-imaging Analyses” Daisuke Saito (Nara Institute of Science and Technology, Japan) |
| 16:45 - 17:00 |
“PRDM14 Promotes Active DNA Demethylation through the Base Excision Repair Pathway in Embryonic Stem Cells” Yoshiyuki Seki (Kwansei Gakuin University, Japan) |
| 17:00 - 17:15 |
“NANOS2-mediated RNA Regulation in the Differentiation of Mouse Male Gonocytes” Atsushi Suzuki (Yokohama National University, Japan) |
| 17:15 - 17:35 | Coffee Break |
| 17:35 - 17:50 |
“Independent Evolution of Sex Chromosomes through Recurrent Use of Sox3 in Medaka Fish and Mammals” Yusuke Takehana (National Institute for Basic Biology, Japan) |
| 17:50 - 18:05 |
“Effect of Fetal Leydig Cell-Specific Ad4BP/SF-1 Gene Disruption on Masculinization and Spermatogenesis” Yuichi Shima (Kyushu University, Japan) |
| 18:05 - 18:20 |
“Y Chromosomal Azoospermic Factor RBMY and Estrogen Signaling Coordinately Regulate Male-specific Alternative Splicing” Matomo Sakari (University of Tokushima, Japan) |
| 18:20 - 18:35 |
“The Germ Plasm-dependent and -independent Mechanisms for the Germ Cell Specification in the Ascidian, C. intestinalis” Maki Shirae-Kurabayashi (Hiroshima University, Japan) |
| 19:00 - 21:00 | Mixer |
| July 19th (Thu) at OCC | |
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The 60th NIBB Conference
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| Session II | Regulatory Mechanism of Germline Sex |
| Mark Van Doren, Peter Koopman | |
| 09:00 - 09:40 |
“Sexual Dimorphism in the Gonad Stem Cell Niche” Mark Van Doren (Johns Hopkins University, USA) |
| 09:40 - 10:20 |
“Mechanism Regulating Germline Sexual Identity in Drosophila Embryos” Satoru Kobayashi (National Institute for Basic Biology, Japan) |
| 10:20 - 10:40 | Coffee Break |
| 10:40 - 11:20 |
“Molecules That Maintain and Regulate Germ Cells during Sexual Differentiation of Medaka Gonads” Minoru Tanaka (National Institute for Basic Biology, Japan) |
| 11:20 - 12:00 |
“Factors Influencing the Sex-specific Differentiation of Fetal Germ Cells” Peter Koopman (The University of Queensland, Australia) |
| 12:00 - 12:40 |
“Genetic Pathways Leading to Sexual Fate Decision in Mouse Germ Cells” Yumiko Saga (National Institute of Genetics, Japan) |
| 12:40 - 13:40 | Lunch Break |
| Poster Session I | |
| 13:40 - 15:30 | Poster Session for odd-numbered posters Poster Session List (pdf 73KB) |
| Session III | Pluripotent Stem Cells and Germline |
| Kazuya Kobayashi, Phillip A Newmark | |
| 15:30 - 16:10 |
“Max is a Repressor of Germ-cell-related Gene Expression in Mouse Embryonic Stem Cells” Yasuhisa Matsui (Tohoku University, Japan) |
| 16:10 - 16:50 |
“Sex-inducing Effects by ‘Feeding’: Switching from Asexual to Sexual Reproduction in the Planarian Dugesia ryukyuensis” Kazuya Kobayashi (Hirosaki University, Japan) |
| 16:50 - 17:30 |
“Germ Cell Development and Regeneration in Planarians:Implications for Understanding Parasitic Flatworms” Phillip A Newmark (University of Illinois, USA) |
| 17:30 - 17:50 | Coffee Break |
| Short Talks II | |
| Yumiko Saga, Minoru Tanaka | |
| 17:50 - 18:05 |
“In Vitro Propagation of Presumptive Germ Cells with Primordial Germ Cell Characteristics Derived from Mouse Induced Pluripotent Stem Cells” Masanori Imamura (Keio University, Japan) |
| 18:05 - 18:20 |
“Acquisition of Pluripotency from Primordial Germ Cells” Go Nagamatsu (Keio University, Japan) |
| 18:20 - 18:35 |
“Nutrition-dependent Control of Ovarian Development by the Fat Body in Drosophila” Hiroko Sano (Kurume University, Japan) |
| 18:35 - 18:50 |
“A Transient Niche for the Germline Stem Cell Precursors in Drosophila Ovary” Miho Asaoka (National Institute of Genetics, Japan) |
| 18:50 - 19:05 |
“t-SNARE Syntaxin2 is Required for Transportation of Glycolipids and Stable Formation of Intercellular Bridges during Spermatogenesis” Yasuhiro Fujiwara (Okayama University, Japan) |
| 19:05 - 19:20 |
“Analysis of Epigenetic Changes Associating with Spermatogonial Stem Cell Differentiation” Kazuyuki Ohbo (Yokohama City University, Japan) |
| July 20th (Fri) at OCC | |
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The 58th NIBB Conference
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| Special Lectures | |
| Robert E Braun, Shosei Yoshida | |
| 09:00 - 10:00 |
“The Germline: Evolution’s Trustee” Allan C Spradling (Carnegie Institution for Science, USA) |
| 10:00 - 10:10 | Coffee Break |
| 10:10 - 11:10 |
“Spermatogonial Stem Cells: Computer Simulation of Their Behaviour in the Stem Cell Niche” Dirk G de Rooij (University of Amsterdam, Netherlands) |
| Short Talks III | |
| Yuzo Niki, Kyle E Orwig | |
| 11:10 - 11:25 |
“Propagation of Nile-tilapia Spermatogonial Stem Cells In Vitro” Samyra Lacerda (Universidade Federal de Minas Gerais, Brazil) |
| 11:25 - 11:40 |
“Establishment of Long-term Culture Conditions for Zebrafish Spermatogonial Stem Cells” Noriyoshi Sakai (National Institute of Genetics, Japan) |
| 11:40 - 11:55 |
“LIF (Leukemia Inhibitory Factor) Promotes Spermatogonial Proliferation in Medaka (Oryzias latipes)” Ryuichi Satoh (Hokkaido University, Japan) |
| 11:55 - 12:10 |
“Low Temperature-induced Circulating T3 Activates Metamorphic Genes and Accelerates Seasonal Testicular Regression” Yusuke Atsumi (Nagoya University, Japan) |
| 12:10 - 13:10 | Lunch Break |
| Poster Session II | |
| 13:10 - 14:50 | Poster Session for even-numbered posters Poster Session List (pdf 73KB) |
| Session IV | Germline Stem Cell Functionality In Vivo |
| Benjamin D Simons, Erika Matunis | |
| 14:50 - 15:30 |
“Stem Cell Renewal in the Drosophila Testis Niche” Erika Matunis (Johns Hopkins University, USA) |
| 15:30 - 16:10 |
“Cellular and Molecular Analyses of a Stable Drosophila Germline Stem Cell Niche Reconstructed In Vitro” Yuzo Niki (Ibaraki University, Japan) |
| 16:10 - 16:30 | Coffee Break |
| 16:30 - 17:10 |
“How are the Self-renewal and Differentiation of Mouse Spermatogenic Stem Cells Balanced?” Shosei Yoshida (National Institute for Basic Biology, Japan) |
| 17:10 - 17:50 |
“The Population Asymmetric Self-renewal of Germ Line Stem Cells” Benjamin D Simons (University of Cambridge, UK) |
| 18:30 - 20:30 | Mixer |
| July 21st (Sat) at OCC | |
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The 58th NIBB Conference
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| Session V | Regulatory Mechanism of Germline Stem Cells |
| Robert E Braun, Takashi Shinohara | |
| 09:00 - 09:40 |
“Functional Compartmentalization of Murine Germ Cells and Translocation of Syncytial Chains across Sertoli Cell Tight Junctions” Robert E Braun (The Jackson Laboratory, USA) |
| 09:40 - 10:20 |
“In Vitro Transformation of Male Germline Stem Cells” Takashi Shinohara (Kyoto University, Japan) |
| 10:20 - 11:00 |
“Translating Spermatogonial Stem Cell Transplantation Toward the Clinic” Kyle E Orwig (University of Pittsburgh, USA) |
| 11:00 - 12:00 | Lunch Break |
| Session VI | Manipulation of Germ Cells |
| Takehiko Ogawa, Goro Yoshizaki | |
| 12:00 - 12:40 |
“Avian Primordial Germ Cell: Its Culture and Manipulation for Transgenesis” Jae Yong Han (Seoul National University, Korea) |
| 12:40 - 13:20 |
“In Vitro Spermatogenesis Using an Organ Culture Method” Takehiko Ogawa (Yokohama City University, Japan) |
| 13:20 - 14:00 |
“Germ Cell Transplantation in Fish: Can Mackerel Produce Tuna?” Goro Yoshizaki (Tokyo University of Marine Science and Technology, Japan) |
| Closing Remarks | |
| 14:00 - 14:10 | Shosei Yoshida & Satoru Kobayashi (National Institute for Basic Biology, Japan) |
