Program |
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| June 28, Sun | ||
| 17:00 | Get together | |
| June 29, Mon | ||
| 09:00 | Welcome address (Kiyotaka Okada, Director General of NIBB) |
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| 09:05 | Introduction of course and Physcomitrella patens | Hasebe and Cuming |
| 09:35 | Spore germination | Hiwatashi |
| Culture and storage of protonemata and gametophores | Hiwatashi | |
| 10:15 | Observation of protonemata and gametophores | Sato |
| Analysis of protonema colony morphology | Sato | |
| Regeneration of protonemata from excised leaves-1 | Sato | |
| 11:05 | Induction of gametangia and sporophytes | Cuming and Kamisugi |
| Sporangium collection and storage | Cuming and Kamisugi | |
| Observation of antheridia and archegonia | Cuming and Kamisugi | |
| Observation of sperms | Cuming and Kamisugi | |
| 12:05 | Lunch | |
| 13:00 | PEG-mediated transformation | Hiwatashi, Cuming and Kamisugi |
| 17:00 | Crosses of mutants defective in the sporophyte formation | Cuming and Kamisugi |
| 18:00 | Dinner | Kurata |
| 20:00 | Workshop 1: Next generation sequencer | Kurata |
| June 30, Tue | ||
| 09:00 | PEG-mediated transformation | Hiwatashi, Cuming and Kamisugi |
| 13:00 | Lunch | |
| 14:00 | Genomic DNA extraction | Sakakibara |
| Chromatin immunoprecipitation | Kurata | |
| RNA extraction | Kurata | |
| Green PCR | Kubo | |
| 15:00 | DNA-gel-blot analysis | Sakakibara |
| RNA interference | Kurata | |
| Artificial micro RNA | Kurata | |
| Gene deletion | Sakakibara | |
| Conditional deletion | Kubo and Sato | |
| Dominant repression | Kubo | |
| Overexpression | Kubo | |
| Inducible expression | Kubo | |
| 18:00 | Dinner | |
| 20:00 | Workshop 2: Targeting strategy | Cuming and Kamisugi |
| July 1, Wed | ||
| 09:00 | PEG-mediated transformation | Hiwatashi, Cuming and Kamisugi |
| 11:00 | How to use genome information | Nishiyama |
| 12:00 | Lunch | |
| Free time / Seimar by Dr. Nir Ohad and Dr. Amal J. Johnston | ||
| July 2, Thu | ||
| 09:00 | How to use genome information | Nishiyama |
| 10:00 | Analysis of protonema development and growth | Sato and Hiwatashi |
| 11:00 | Observation of microtubules with indirect immunofluorescence microscopy | Murata |
| Light and electron microscopy of protonemata embedded with epoxy resin | Murata | |
| 12:00 | Lunch | |
| 13:00 | DAPI, Hoechst33342, and PI staining of protonemata | Murata |
| Visualization of cell wall with calcoflur | Murata | |
| 14:00 | Live imaging | Sato |
| Cellular localization of fluorescent protein | Sato | |
| Regeneration of protonemata from excised leaves-2 | Sato | |
| 16:00 | GUS staining | Hiwatashi |
| 17:00 | How to use genome information | Nishiyama |
| 18:00 | Dinner | |
| 20:00 | Workshop 3: Bioimaging | Sato, Murata, Cuming and Kamisugi |
| July 3, Fri | ||
| 09:00 | Measurement of DNA content with FACS | Sato |
| 10:00 | Agrobacteria-mediated moss transformation: Protonema and Agrobacteria Co-culture |
Kurata |
| 12:00 | Lunch | |
| 13:00 | Agrobacteria-mediated moss transformation: Protonema and Agrobacteria Co-culture |
Kurata |
| 14:00 | Hormone treatments | Cuming and Kamisugi |
| 15:00 | Light response | Sato |
| Gravitropic response | Sato | |
| 16:00 | Closing discussion | All lecturers |
| 17:00 | Farewell party | |
 Copyright(C) National Institute for Basic Biology. All rights reserved. |
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