ISH photos


List of photos

  • <Single ISH>
    • > Nurr1 in monkey cortex
    • > ER81, 5-HT2C receptor, Nurr1 and CTGF mRNAs at V1-V2 border
    • > Nurr1 and CTGF mRNA expression in mouse cortex
    • > ER81 mRNA expression in monkey brains (movie)
      > RBP/occ1 mRNAs in monkey cortex
  • <Double ISH>
    • > ER81/VGluT1 genes in mouse barrel cortex
      > ER81/VGluT1 genes in monkey area TE
    • > CTGF/Nurr1 mRNA in mouse cortex
    • > Nurr1/VGluT1 mRNAs in monkey area V2
    • > Parvalbumin/GAD67 or VGluT1 mRNAs in mouse cortex
    • > occ1/PV mRNAs in mokey motor area
    • > RBP/VGluT1 or GAD67 mRNAs in monkey cortex
  • <Triple ISH>
    • > CTGF/ER81/Nurr1 in layer 6 of monkey motor cortex

  • <For more>
    • What is neocortex?
    •     A photo guide of neocortical structure revealed by gene expression
    • Brain Atlas
    •     Serial sections of monkey and mouse brains stained for variuos genes


<Single ISH>

> Nurr1 in monkey cortex
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Nurr1 mRNA is expressed in a subpopulation of layer 6 neurons througout monkey neocortical areas.  It is also strongly expressed in claustrum (Cl) and substantia nigra (SN), which features are conserved between monkeys and mice.  Visual cortex (V1) of monkeys has many features that distinguish it from other cortical areas.  In this photo, you can see the segregation of Nurr1 mRNA stripe into two substrata only in V1 (see below for magnified view).  For more info, see Watakabe et al. 2006 Cereberal Cortex.




> ER81, 5-HT2C receptor, Nurr1 and CTGF mRNAs at V1-V2 border in monkeys

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Expression of four genes at the border between the primary and secondary visual cortex (V1 and V2) of monkeys.  Monkeys generally have a very good vision, which is manifested in well-developed V1.  The layer specific genes shown here exhibit the clear-cut border between V1 and V2, consistent with the specilization of V1.  At the same time, it is also evident that the basic lamina structure is conserved even across this sharp border.  See Watakabe et al. 2006 Cereberal Cortex.




> Nurr1 and CTGF mRNA expression in mouse cortex

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There are two modes of Nurr1 mRNA expression in mouse cortex.  Expression in layer 6a is confined to lateral areas, whereas expression in lyaer 6b is observed across entire neocortex.  The latter pattern is similar to that of CTGF mRNA.  The double ISH showed that these two mRNAs are co-expressed in layer 6b (see below).  See Watakabe et al. 2006 Cereberal Cortex.




> ER81 mRNA expression in monkey brains (movie)
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Serial sections of monkey brain stained for ER81.  Quicktime movie (696kB)


Movie download





> RBP/occ1 mRNAs in monkey cortex
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RBP and occ1 exhibit highly area-specific expression patterns in monkey cortex.  These two genes exhibit complementary area profiles.  occ1 gene is enriched in visual cortex and is barely expressed in the frontal areas.  On the other hand, RBP is abundant in the association areas in the frontal and temporal areas, whereas it is barely expressed in the visual cortex. YaYIt When



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<Double ISH>

> ER81/VGluT1 genes in mouse barrel cortex
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Double ISH of ER81 (layer 5- "specific") and VGluT1 (excitatory marker) genes in mouse barrel cortex (somatosensory area for whiskers).  ER81 is almost specific to layer 5 in mouse or rat cortex.  Note that ER81 positive neurons are a subpopulation of VGluT1-positive exitatory neurons.    See Watakabe et al. 2006 Cereberal Cortex.




> ER81/VGluT1 genes in monkey area TE
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Double ISH of ER81 (layer 5- "specific") and VGluT1 (excitatory marker) genes in monkey area TE.  Unlike in mouse or rat cortex, ER81 mRNA is expressed by a select subpopulation of layer 6 neurons in monkey cortex in addition to layer 5.  Note that ER81 positive neurons are a subpopulation of VGluT1-positive exitatory neurons (see below for magnified view).    See Watakabe et al. 2006 Cereberal Cortex.


........Magnified view of the above photo







> CTGF/Nurr1 mRNA in mouse cortex
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Double ISH of CTGF and Nurr1 genes at the border between primary and secondary somatosensroy areas in mouse cortex.  Note that Nurr1 positive neurons in layer 6b co-expresses CTGF mRNAs.    See Watakabe et al. 2006 Cereberal Cortex.




> Nurr1/VGluT1 mRNAs in monkey area V2
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Double ISH of Nurr1 and VGluT1 (excitatory marker) genes in monkey area V2.  Note that Nurr1 positive neurons are a subpopulation of VGluT1-positive exitatory neurons.    See Watakabe et al. 2006 Cereberal Cortex.





> Parvalbumin/GAD67 or VGluT1 mRNAs in mouse cortex
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Double ISH of parvalbumin (PV) and GAD67 (inhibitory marker) or VGluT1 (excitatory marker) genes in mouse cortex.  PV positive neurons consititute a distinct subpopulation of GABAergic inhibitiory neurons.  Note the co-expression of PV and GAD67, but not with VGluT1.  Unpublished material.  




> occ1/PV mRNAs in mokey motor area
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occ1 mRNA is highly enriched in V1 in monkey cortex.  However, it is expressed also by a subpopulation of GABAergic interneurons across cortical areas.  Parvalbumin is a well-known marker for a subtype of GABAergic neurons.  You can observe extensive co-expression of occ1 and PV mRNAs.  See Takahata et al. 2006 Cereberal Cortex.



> RBP/VGluT1 or GAD67 mRNAs in monkey cortex
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RBP exhibits area-specific lamina profiles.  In the frontal and temporal association areas, it is highly exprssed by excitatory neurons across layers.  RBP tends to be expressed in more superficial layers as the area becomes "lower-order".  In this photo, double ISH of RBP with VGluT1 (excitatory marker) or with GD67 (inhibitory marker) in area 1, the primary somatosensory area in monkey cortex is shown.  Note that RBP is expressed strongly in layer 2 excitatory neurons, moderately by layer 3 neurons in the superficial part, but not expressed by excitatory neurons in the lower tier.   See Komatsu et al. 2005 Cereberal Cortex.



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<Triple ISH>

> CTGF/ER81/Nurr1 in layer 6 of monkey motor cortex




ER81, Nurr1 and CTGF mRNAs are enriched in layers 5, 6 and 6b in monkey cortex.  They are co-expressed in many cells in layer 6b. See Watakabe et al. 2006 Cereberal Cortex.

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