NATIONAL INSITUTE FOR BASIC BIOLOGY

National Institute for Basic Biology

CENTER FOR ANALYTICAL INSTRUMENTS

(Managed by NIBB)

Head of Facility:
Tetsuo Yamamori
Technical Staffs:
Akio Murakami (-Apr. 30, 1998)
Sonoko Ohsawa
Tomoko Mori (Jun.1, 1998 -)
Yumiko Makino
Takeshi Mizutani
Technical Assistant:
Hatsumi Moribe


The Center, equipped with various types of instruments, serves for general use and supports biochemical structural analyses of biomolecules of proteins, nucleic acids and lipids. Amino acid sequence analysis, amino acid analysis, and chemical syntheses of peptides and DNA/RNA are carried out to support researchers in NIBB and NIPS. On June 1 in 1998, the Center merged with the facility of Molecular Biological Analysis in the Laboratory of Gene Expression and Regulation. Consequently, instruments transferred to the Center are Particle Delivery System, DNA Sequencers, DNA Synthesizer, Plasmid Isolation Systems, etc. Newly installed instruments are Fluorescence Bio Imaging Analyzer and DNA Sequencer (ABI 310). Some of instruments of the Center are opened to researchers outside the Institute.

Representative instruments are listed below.

Figure 1.
Amino acid sequence analysis of protein by protein sequencer.

Figure 2.
DNA sequencing electropherogram.


Protein Sequencers (ABI 494, ABI 473A)
Amino Acid Analyzer (Hitachi L8500A)
Peptide Synthesizers (ABI 433A, ABI 432A)
Plasmid Isolation Systems (Kurabo PI-100 S)
DNA Sequencers (ABI 377, 373S, ABI 310)
DNA/RNA Synthesizers (ABI 394, ABI 392)
Thermal Cyclers (Perkin Elmer PJ-9600, Takara TP-300)
Integrated Thermal Cyclers (ABI CATALYST Turbo 800)
Particle Delivery System (Bio-Rad BiolisticPDS-1000/He)
Gas Chromatograph (Shimadzu GC-14APF-SC)
Glycoprotein Analysis System (Takara Glyco-Tag)
High Performance Liquid Chromatographs (Shimadzu LC-10AD, 6AD)
Integrated Micropurification System (Pharmacia SMART)
Flow Cytometer (Coulter EPICS XL)
NMR Spectrometer (Bruker AMX-360wb)
EPR Spectrometer (Bruker ER-200D)
GC/Mass Spectrometer (JEOL DX-300)
Inductively Coupled Plasma Atomic Emission Spectrometer (Seiko SPS1200A)
Spectrofluorometers (Hitachi 850, Shimadzu RF-5000)
Spectrophotometers (Hitachi 330, Hitachi 557, Varian Cary 5G, Perkin Elmer Lambda-Bio)
Microplate Luminometer (Berthold MicroLumat LB 96P)
Time-resolved Fluorescence Microplate Reader (Pharmacia DELFIA Research)
Microplate Readers (Corona MTP-120, MTP-100F)
Spectropolarimeter (JASCO J-40S)
FT-IR Spectrophotometer (Horiba FT-730)
Laser Raman Spectrophotometer (JASCO R-800)
Biomolecular Interaction Analysis Systems (Pharmacia BIAcore 2000, Affinity Sensors IAsys)
Bio Imaging Analyzers (Fujifilm BAS2000)
Fluorescence Bio Imaging Analyzer (Takara FMBIO II)
Electrophoresis Imaging Systems (PDI Discovery Series, BIOIMAGE)
Microscopes (Carl Zeiss Axiophot, Axiovert)
Microscope Photometer (Carl Zeiss MPM 03-FL)


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Last Modified: 12:00, May 28, 1999