NATIONAL INSTITUTE FOR BASIC BIOLOGY
Fig. 2. SGF-1 interaction with SA site.
Mildly methylated DNA was used as a probe for gel shift assay. DNA extracted from free probe (F) or retarded band (R) was cleaved and resolved in a sequencing gel. A. Footprint of the coding strand using pure 42 kDa protein. B. Footprint of the noncoding strand using protein fraction obtained after DNA affinity resin. C. SA site in the extend previously identified by DNaseI footprinting. G and A residues the methylation of which actually affect SGF-1 binding are indicated in bold. These residues are marked by arrowheads in A and B.