Training

Light Microscopy

Research Supporter :Yasushi Hiraoka
Graduate School of Frontier Biosciences, Osaka University

Course Objectives
Lectures and practical courses of fluorescence microscopy techniques to analyze localization and mobility of intracellular molecules in living cells. Principles and practices of fluorescence microscopy will be provided.

Course Topics/Modules
Principles of microscopy, measurements of the point-spread function, introduction of fluorescent reagents into the cell, observation of living cells, time-lapse imaging using wide-field and confocal microscopes, measurements of protein mobility by FRAP, FLIP, photoactivation in living cells

Audience
About 16 Graduate students and 4 postdocs/young researchers

Location
Advanced ICT Research Institute Kobe, National Institute of Information and Communications Technology 588-2 Iwaoka, Iwaoka-cho, Nishi-ku, Kobe 651-2492

Web site
http://www2.nict.go.jp/advanced_ict/bio/w131103/CellMagic/

  • Wide-field microscope system
    ・ DeltaVision (GE/Corns)

  • Confocal microscope system
    ・ LSM800 (Zeiss)
    ・ FV1200 (Olympus)
    ・ A1 (Nikon)
    ・ SP8 (Leica)
    ・ CSU-W1 (Yokogawa)